Abnova is a company in Taiwan, claiming they are "the World's Largest Monoclonal Antibody and Recombinant Protein Manufacturer".
I have to admit that it feels exciting when you see a company from your homeland competing with other big-names on the worldwide market. I hope they are doing well. Just think about how wonderful it will be if labs in Taiwan can get their favorite antibodies in days, but not for weeks waiting for the ocean-corssing shipping arrived.
This is what written on their website:
Abnova is the world largest monoclonal antibody manufacturer. We have a capacity of generating 500 mouse monoclonal antibodies per month. Rather than the traditional method of monoclonal antibody production, Abnova is taking a genomic/proteomic approach for the antibody development. Our goal is to have at least one antibody to every human expressed gene in human genome. We manufacture all our products, recombinant proteins and antibodies, in house in our stat-of-art facility in Taiwan. In most cases, we have multiple clones for our monoclonal antibodies, and their access is available to our industrial partners and collaborators from the academic institutions.
Maybe the bio-industries still have their shots in Taiwan, if they find the niche and have the foresight and sagacity.
Then I started to recall that I ever emailed them inquiring another antibody. Never got reply.
I just sent another email asking information about the three anti-Cep55 antibodies. Hope the customer service is improved this time!
12 comments:
You should ask for aliqouts from "here".
I have Abnova's Cep55 antibody. It doesn't work well in IF. But if you are using it for WB, I don't know. If you can work out the staining condition, plz let me know. Shipping fee is notoriously expensive, but your lab shouldn't mind.
Kun Kun Khuma's Cep55 antibody can be used in Methanol fixed and tolerate short and low conc. of aldehyde fixation
Fang's Cep55 antibody works beautifully but very sensitive to aldehyde-fixation
Another German lab also creates Cep55 antibody. They are only willing to share serum with us, but they do have AP-Cep55 antibody. Maybe you will have a better luck.
-Ting
Ha....I read your article again. You are just googling....
Our lab bought two antibodies from Abnova. One, as you know, is Cep55; the other is centrin. Neither works well....so....we don't have much faith in this company...
-Ting
I emailed Khuma asking for the GFP-Cep55 and anti-Cep55 antibody. Have not gotten any response. Am I asking to much? ha-ha.
For Adnova's Cep55 antibody, a Science article used their monoclonal anti-Cep55 antibody (A01) and the immunofluorescence figures looked okay.
The reason I am interested in Cep55 is so far it is the only protein I know can make the black-zone in the middle of midzone goes away. I am wondering if it has anything to do with the electron dense material in that region. Some experts in Woods Hole think based on EM pictures, those electron dense materials should not be (only) proteins. Some guess it is RNA but I think it is not very likely.
I will definetely consider your suggestion!
Ha, I kind of dislike you now. XD
One of the subaim we proposed in KECK grant is to conditionally KO-Cep55 in mice. The idea is the same as you said here, because Cep55 is the only known protein that has been shown to be able to disintegrate midbody or, probably, its remnant.
FYI, we compared the distribution of Mklp1 and Cep55-GFP (or Mklp1-eGFP and Cep55) in that dark zone. I would say Cep55 occupies more core-like region in the dark zone. Cep55 and Mklp1 have very intriguing and different topological distribution at the midbody matrix during cytokinesis.
Great, I will check out that Cep55 paper. I guess you are talking about the paper describing Alix and Tsg proteins for abscission. Right?
The words from Fabbros are that Khumma is not interested in Cep55 right now. She is. So, maybe you should ask her instead to have this antibody.
Fang has a lot. The first AP and conc. aliqout he shared is so good, but at that time we were not aware of how precious it is....Later, he is only willing to give us the AP, but not conc. version of aligout. It dies so fast; now I have to use 1:10 (the first aliqout can be used at 1:1000)
I thought you are working on furrow stuff, rather than post-ingression stuff? Changing scope?
Welcome to join midbody club! Steve will present some (or most of ) our data in the minisymposium at Harvard U.
-Ting
I don't think I will "join" the midbody field. :)
Instead, I am more interested in the earlier stage before the furrow ingression completes.
I believe there is a mechanism to "stabilize" equatorial microtubules. PRC1 bundling probably plays some role in it but I think the microtubule plus ends are somehow modified to be stable.
And I suspect cortex does similar things on the equatorial aster microtubules too.
Say it more precisely, I am interested in microtubule stabilization in cytokinesis rather than midbody integration. That is why I like to test the Cep55. As you can tell by many published data, the block zone (electron dense material) appears way earlier in midzone stage than the late midbody formed.
One thing bothers me is, when you ask reagents from someone, will you give then your fedex account in the first mail so they can send it to you directly?
I usually give them our fedex account when I got their reply. But there are about 50% of the case I never get any response. It's so wierd.
Ha, My successful rate is much higher than yours....
But, I don't have a quick response in the first email, either. If that is the case, I will email again (one or two weeks) and say something like "you must be busy and miss my previous email" or "I was unable to be reached last week and may miss your response" etc. A lot of times, people will email back after my second email. After people email us back, we will tell them our fedex number.
sometimes I email to the first author and the corresponding author if it seems like the first author is still in the lab.
If it still doesn't work, Steve will do his job: the third email.
As for the subject you are interested, I think I have seen that (we called it double rings) before either by Cep55 or some other midbody components.
-Ting
I am not sure. I saw the electrodense material even before the furrow ingression starts. There should be no "double rings" in that stage I think.
BTW, how do you define a mature midbody (stem body)? by what molecular marker?
Yeah, consistency is the only way to deal with those PIs.
I emailed Steve this morning because I want to make sure he will agree what I have promised you though I don't think he would disagree to share.
He told me that I should give to you whatever you want. So, you shall be able to receive package at Fri.
There is only one problem. The email you sent to me earlier including Fedex number is lost during my reinstallation of email client software. Can you do that again and provide address for us?
-Ting
Have you got a reply from Abnova now?
to Ting,
Thanks, I don't have those info in hands right now at home. I will email you tomorrow in the lab.
By the way, Steve said you to give me whatever I want. As you shall know, "whatever" is a very dangerous term...hee...
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To the anonymous who asked if I received any reply fro Abnova.
Are you the coworker of Ivy Hung? Yes, I do have received your reply by email, twice. Thank you very much.
FYI, the one I mentioned in my article which didn't get any reponse from abnova, is not referring to anti-Cep55. I forget what that antibody is, since it happened about one year ago.
So, are you an employee in Abnova? Impressive...
Trying to establish personal connection.....Wow! What a good customer support!
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Let me quote what Steve has said to me: "In the meantime we should get him whatever he wants primarily because he is in Tims lab. I overlapped with Tim when we were postdocs in the kirschner lab."
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Somehow I found a way to retrieve your emails. I have the info I need. If you can have AP-antibody from that Germany lab and the volume of the antibody is ok for sharing, I want to have some in return.
-Ting
I sent an email to your gmail account.
Sure, no problem.
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