JCB 15:45 (2002/10)
Yu-li Wang @ UMMS
This paper took me two days. It is not because this paper is especially difficult or what. I just couldn't help but kept fallen asleep while reading it. I always like Yu-li's paper because of their micromanipuation experiments. However I just don't understand why they had not published any paper in top profile journals these years? Is that because their papers only give a descriptive conclusion but no detailed mechanism?
Anyway, in this paper, authors performed the FRAP experiment for Aurora B on different parts of the cell at different stages. It showed that Aurora B has a very slow turnover rate along midzone microtubules which is in contrast to before cytokinesis on kinetochores. Authors also suggested that Aurora B was directly relocating from centromeres to midzone microtubules. It disagrees with later findings that you don't need chromosomes to localize Aurora B on midzone microtubules. Aurora B also localized on astral microtubules and at least it suggested kinetochores/centromeres are not the only entry point for Aurora B on microtubules. Astral microtubules could transport Aurora B to equatorial cell cortex where the cleavage will be.
Authors thought midzone and astral microtubules might play their roles in different stages such as late and early cytokinesis. But I don't buy it. Is that clear what is different between midzone and astral microtubules before the former got stabilized?
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