Hmm... Does anyone here happen to know what is the epitope of the monoclonal anti-GFP (3E6) antibody?
3E6 is a monoclonal anti-GFP antibody wildly used. We would like to use it for protein purification of the GFP-fusion proteins from mammalian cell lysate. The problem is, we need the blocking peptide to compete and elute the bound GFP-fusion protein from the antibody. Does anyone know the epitope sequence of 3E6?
I spent my whole afternoon googling and found nothing... sigh.
I also sent emails to three companies selling this antibody; hopefully someone will answer me.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment