Finally I got the cell I wanted badly, the hybridoma for anti-GFP antibodies.
I was looking for good monoclonal anti-GFP antibodies for IP, and found a paper from China. I emailed them and later was informed they were leaving for the epidemic prevention in Sichuan Province where the horrible earthquake took place. After their return, they were very nice to me, and eventually said yes to give me their hybridoma cells. I told them that I came from Taiwan and felt very sorry about the earthquake. Possibly the significant donation from Taiwan really warms their hearts. It is really a big favor for giving away the hybridoma cells for nothing. I truly appreciate it.
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Good thing to learn that you got this cell line. We purchased it from commercial and it was quite expansive.
Yeah.. I am quite happy about it.
There are not many good anti-GFP mAbs for IP. Most good ones are polyclonal.
Invitrogen 3E6 mAB is excellent, but frequently backordered for up to 6 months (I am sure 3E6 binds to a continuous GFP domain and can be blocked by the corresponding peptide; however it is extremely difficult to dig out related information from those companies).
Since what I got is a different line established by the chinese group. My next step is to figure out its epitope domain. Hopefully it recognizes a linear domain, not a folded 3-D structure. Then I can use this blocking peptide to elute the antibody-bound GFP-fusion proteins, with activities.
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