花栗鼠站出來

昨天看到凡凡的能耐，覺得 crib 的柵欄大概低了些，可能凡凡腳用力一蹬就可以翻牆而出，所以今天動手將 crib 的床面放低，想這樣應該就安全些了。凡凡習慣先抓柵欄的上緣再用力起身，改成這樣大概一時還不能適應，一開始頭就撞了一下，不過好像沒事一樣，大概不會痛的樣子。努力一陣果然又成功了，而且可抓的地方較高，兩腳可以站得很直，站得更穩。大概是很開心吧，凡凡後來還想去打開 mobile 來展一下他有多能幹。



站穩後就發現這高度剛好，正適合磨牙，所以就表演花栗鼠的動作給大家看看。

度咕

在餐廳等吃的等太久了，等到都打起瞌睡來了，不過湯匙還是得抓好，免得等一下有得吃的時候反而吃不到，那就虧了。

覓食

現在真的很有體力也很能動，能爬的地方就爬，能站的地方就站，能抓的東西就抓，能吃的東西就吃，不能吃的東西也是照吃。只是奶瓶太大手太小不容易抓穩，加上正在打嗝，一不小心就失去平衡，不過，摔倒事小，到手的奶瓶當然還是得死抓著不放啊。

Cooperation between Mitotic Kinesins Controls the Late Stages of Cytokinesis

Current Biology 16:301 (2006/02)
Francis Barr @ Max-Planck-Institute of Biochemistry

There are two possible Aurora B phosphorylation sites in MKLP1, S821 and S911. Due to the failure in generating phospho-S821-specific antibody, only S911 was addressed in this paper. Authors showed that Aurora B phosphorylates MKLP1 S911 during anaphase. The S911A mutant did not cause any defect in early stages of cytokinesis, including contractile ring build-up and furrow ingression. However, this phosphorylation on S911 is required for MKLP1 stay at the midzone/midbody during late cytokinesis. A bipartite NLS in MKLP1 was disrupted by S911 phosphorylation so MKLP1 will not be recruited back to nucleus after nuclear membranes reformed.

Plk1, Cdk1, and Aurora B were all tested and only Aurora B could phosphorylate MKLP1 S911 both in vitro and in vivo. Besides, MKLP2 is required for this phosphorylation since it regulates the translocation of Aurora B in cytokinesis. Unfortunately authors didn’t address how the mislocalization of MKLP1 causes cytokinesis failure during late stages. The only evidence authors had is a increase of binucleate cell percetage. It is just not solid enough.

Unlike vertebrates and Drosophila, C. elegans appears to lack MKLP2. Besides, S911-containing NLS region in C-terminus of mammalian MKLP1 is absent from the C. elegans homolog ZEN-4. It suggests this regulatory mechanism is specific for vertebrates and either does not occur in C. elegans or involves a different mechanism. The second site, S812 in the tail region downstream of the actin binding domain, is conserved in ZEN-4, where it is required for regulation of the late stages of cytokinesis. Whether MKLP2 is also required for S812 phosphorylation in human cells and for other Aurora B substrates such as Cyk-4 (MgcRacGAP), is still unknown.

凡凡站出來

凡凡已經可以藉由扶或抓著東西把自己給拉起來了，還能好好穩穩地站著。

又見跳躍男孩！

凡凡長大也強壯了許多，做在吊椅上已經可以停下來站好東張西望，還可以歪頭探看他媽媽在做甚麼，不用像穿了紅舞鞋一樣一直跳不停沒法停下來了。

匍匐前進

很奇妙地，昨天凡凡還不會爬行，今天忽然就會了，不知哪時哪裡學來的？看起來雖然手腳還不是很協調，離革命軍人戰鬥教練三行四進的標準還是很遠，不過總還算是個開始就是啦！

翻來覆去

凡凡現在的力氣與肢體協調性都越來越好了，嘗試後讓他發現訣竅就會記得，像現在他已經能隨意轉換爬行與坐著兩種不同的姿勢了。不過有可能是因為頭太大顆了，有的時候還是會重心不穩栽下去。

I Like the Way

這歌在去年底是可口可樂的電視廣告歌，是我覺得畫面和音樂配合的最好的可樂廣告。有時要寫東西或者騎車時會拿來聽，提振一下精神。

I Like the Way / Bodyrockers
MV version

Live version


There's so many things I like about you / I / I just don't know where to begin / I like the way you look at me with those beautiful eyes / I like the way you act all surprised / I like the way you sing along / I like the way you always get it wrong / I like the way you clap your hands / I like the way you love to dance / I like the way you put your hands up in the air / I like the way you shake your hair / I like the way you like to touch / I like the way you stare so much / But most of all / Yeah / Most of all / I like the way you move / I like the way you move

I like the way you put your hands up in the air / I like the way you shake your hair / I like the way you like to touch / I like the way you stare so much / But most of all / Yeah / Most of all / I like the way you move / I like the way you move

I like the way you put your hands up in the air / I like the way you shake your hair / I like the way you like to touch / I like the way you stare so much / But most of all / Yeah / Most of all / I like the way you move / I like the way you move

Black TGIF

This week, it is our lab's turn to host the happy hour party.
Kueh made a brilliant flyer for it.

Darth Tater: Your microtubule is short, old man.
Mr.Potato: Size matters not. Dynamic instability does.